Ardour exposes multiple ports for various parts of the signal chain to link those parts: track inputs and outputs, bus inputs and outputs, sends and inserts, monitor section outputs. When using the JACK audio backend, these ports are also accessible by other applications and can be routed externally.

The chart on the right demonstrates a common signal flow for recording an instrument: a guitar is plugged into a front input of an audio interface, the signal then goes directly into the track output, passes the processor box with plugins, fader, and panner, connects to the input of the master bus, passes its processor box, the goes into the monitor section, then finally connects to physical outputs like studio monitors or headphones.

This configuration can have multiple variations, such as:

• There can be a DI box sitting between the guitar and the front input, if the guitar has a passive pickup.
• The signal from a guitar can pass a DI box and feed into one track, but another path can go through preamp/amp/cabinet/mic and feed into another track, so that the musician has both processed sound and dry that can be re-amped later on.
• The same can be achieved by creating an input I/O plugin (a guitar amp/cab simulator), passing a copy of guitar's DI'ed signal through it and feeding the I/O plugin's output to another track.
• Monitoring could be done with hardware, so that there would be no monitor section, and thus the master bus would be connected to physical output ports directly.

When Ardour creates multiple tracks and/or busses at once, this is what happens.

• Track inputs are optionally auto-connected to hardware inputs, in the round-robin order. In the example below where an audio interface only has two inputs and 8 new tracks have been created, Ardour connects the first input to the first track, then the second input to the second track, then the first input to the third track, and repeats it until all tracks have an input assigned for them. The exact configuration will depend on how many channels have been chosen for each new track in the Session > New Session dialog.

  

• Bus inputs are left disconnected.
• The number of track and bus outputs are equal to the number of inputs of the master bus.
• Track and bus outputs are always auto-connected to the master bus inputs.
• Master bus outputs are connected to hardware outputs if new session don't have a monitor section by default (this is set when the user runs Ardour for the first time and can be changed on the Monitoring Page of the Preferences dialog).

This configuration is sufficient to do basic tracking and playback of many sessions without any adjustment by the user. Changing these connections is generally not necessary and often leads to problems.

However, for many workflows during mixing, more complicated signal routing is required. Ardour offers many possibilities for connecting things to fit any particular workflow.