diff --git a/libs/ardour/audio_playlist.cc b/libs/ardour/audio_playlist.cc
index aa67bca868..e080d1b8ec 100644
--- a/libs/ardour/audio_playlist.cc
+++ b/libs/ardour/audio_playlist.cc
@@ -39,8 +39,8 @@ using namespace std;
 using namespace PBD;
 
 #include "ardour/audioengine.h"
-#define S2SC(s) Temporal::samples_to_superclock (s, AudioEngine::instance()->sample_rate())
-#define SC2S(s) Temporal::superclock_to_samples (s, AudioEngine::instance()->sample_rate())
+#define S2SC(s) Temporal::samples_to_superclock (s, TEMPORAL_SAMPLE_RATE)
+#define SC2S(s) Temporal::superclock_to_samples (s, TEMPORAL_SAMPLE_RATE)
 
 AudioPlaylist::AudioPlaylist (Session& session, const XMLNode& node, bool hidden)
 	: Playlist (session, node, DataType::AUDIO, hidden)
diff --git a/libs/ardour/audioregion.cc b/libs/ardour/audioregion.cc
index b9b33c93de..e33d13d217 100644
--- a/libs/ardour/audioregion.cc
+++ b/libs/ardour/audioregion.cc
@@ -70,8 +70,8 @@ using namespace std;
 using namespace ARDOUR;
 using namespace PBD;
 
-#define S2SC(s) Temporal::samples_to_superclock (s, AudioEngine::instance()->sample_rate())
-#define SC2S(s) Temporal::superclock_to_samples (s, AudioEngine::instance()->sample_rate())
+#define S2SC(s) Temporal::samples_to_superclock (s, TEMPORAL_SAMPLE_RATE)
+#define SC2S(s) Temporal::superclock_to_samples (s, TEMPORAL_SAMPLE_RATE)
 
 namespace ARDOUR {
 	namespace Properties {
diff --git a/libs/ardour/ltc_slave.cc b/libs/ardour/ltc_slave.cc
index d29e96cec0..21968e28d7 100644
--- a/libs/ardour/ltc_slave.cc
+++ b/libs/ardour/ltc_slave.cc
@@ -151,6 +151,7 @@ ARDOUR::samplecnt_t
 LTC_TransportMaster::update_interval() const
 {
 	if (timecode.rate) {
+		/* Use engine rate, timecode ports are not resampled */
 		return AudioEngine::instance()->sample_rate() / timecode.rate;
 	}
 
diff --git a/libs/ardour/lv2_plugin.cc b/libs/ardour/lv2_plugin.cc
index 5acf0d0eb3..4cef12aee4 100644
--- a/libs/ardour/lv2_plugin.cc
+++ b/libs/ardour/lv2_plugin.cc
@@ -2747,12 +2747,12 @@ LV2Plugin::connect_and_run(BufferSet& bufs,
 	samplepos_t start0 = std::max (samplepos_t (0), start);
 
 	TempoMap::SharedPtr tmap (TempoMap::use());
-	TempoMetric metric (tmap->metric_at (samples_to_superclock (start0, AudioEngine::instance()->sample_rate())));
+	TempoMetric metric (tmap->metric_at (samples_to_superclock (start0, TEMPORAL_SAMPLE_RATE)));
 
 	TempoMapPoints tempo_map_points;
 	tmap->get_grid (tempo_map_points,
-	                samples_to_superclock (start0, AudioEngine::instance()->sample_rate()),
-	                samples_to_superclock (end, AudioEngine::instance()->sample_rate()), 0);
+	                samples_to_superclock (start0, TEMPORAL_SAMPLE_RATE),
+	                samples_to_superclock (end, TEMPORAL_SAMPLE_RATE), 0);
 
 	if (_freewheel_control_port) {
 		*_freewheel_control_port = _session.engine().freewheeling() ? 1.f : 0.f;
@@ -2890,9 +2890,9 @@ LV2Plugin::connect_and_run(BufferSet& bufs,
 					}
 				}
 
-				while (m != m_end || ((tempo_map_point != tempo_map_points.end()) && ((*tempo_map_point).sample(AudioEngine::instance()->sample_rate()) < tend))) {
+				while (m != m_end || ((tempo_map_point != tempo_map_points.end()) && ((*tempo_map_point).sample(TEMPORAL_SAMPLE_RATE) < tend))) {
 
-					if (m != m_end && ((tempo_map_point == tempo_map_points.end()) || (*tempo_map_point).sample(AudioEngine::instance()->sample_rate()) > (*m).time())) {
+					if (m != m_end && ((tempo_map_point == tempo_map_points.end()) || (*tempo_map_point).sample(TEMPORAL_SAMPLE_RATE) > (*m).time())) {
 
 						const Evoral::Event<samplepos_t> ev (*m, false);
 
@@ -2905,7 +2905,7 @@ LV2Plugin::connect_and_run(BufferSet& bufs,
 
 					} else {
 						assert (tempo_map_point != tempo_map_points.end());
-						const samplepos_t sample = tempo_map_point->sample (AudioEngine::instance()->sample_rate());
+						const samplepos_t sample = tempo_map_point->sample (TEMPORAL_SAMPLE_RATE);
 						const Temporal::BBT_Time bbt = tempo_map_point->bbt();
 						double bpm = tempo_map_point->tempo().quarter_notes_per_minute ();
 
diff --git a/libs/ardour/session_vst.cc b/libs/ardour/session_vst.cc
index 805d65450e..697a1e3b8c 100644
--- a/libs/ardour/session_vst.cc
+++ b/libs/ardour/session_vst.cc
@@ -280,7 +280,7 @@ intptr_t Session::vst_callback (
 
 		} else {
 			timeinfo->samplePos = 0;
-			timeinfo->sampleRate = AudioEngine::instance()->sample_rate();
+			timeinfo->sampleRate = TEMPORAL_SAMPLE_RATE;
 		}
 
 		if ((timeinfo->flags & (kVstTransportPlaying | kVstTransportRecording | kVstTransportCycleActive))